Dodecyl Sulfate Polyacrylamide Gel Electrophoresis of Proteins in Liver Ribosomes.
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چکیده
منابع مشابه
High-temperature sodium dodecyl sulfate polyacrylamide gel electrophoresis.
The run time for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be reduced to as little as 5 min by running vertical slab gels at 70°C. High-temperature SDS-PAGE increases the migration rate of protein bands several fold and is as effective as cooling in preventing uneven migration rates across the width of the gel. Additional benefits of hightemperature SDS-PAGE inclu...
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In this report we describe a novel approach to the mass spectrometric analysis of whole proteins from gels. The strategy consists of three components: conventional SDS-PAGE gels, reversible negative staining procedures, and passive elution of proteins from gels followed by mass spectrometric analysis. Protein bands are excised from SDS-PAGE gels, destained, and extracted. For gel loadings > or ...
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ژورنال
عنوان ژورنال: The Journal of Kansai Medical University
سال: 1974
ISSN: 0022-8400
DOI: 10.5361/jkmu1956.26.4_516